Figure 6

Time course of phospho-protein induction by TNFα, 2GF, or a combination in FLS. (a). Representative Western blot scans. GAPDH was used as loading control. (b)-(f). Ratios between phospho- and total MAPK and Akt as determined by densitometry. The two ERK isoforms were analyzed together (e). Data are normalized to 2GF or TNFα alone at 15 minutes, as indicated. Mean & SEM, n = 3 RA FLS lines. Asterisk indicates P < 0.05 to 2GF+TNFα by two-way ANOVA and contrast testing.