Figure 7

Involvement of PI3 kinase, but not Erk, in 2GF potentiation of TNFα-induced IL6 gene expression by FLS. (a). FLS were pre-treated with PD98059 (20 μM) or LY294002 (40 μM) 30 minutes prior to 2GF and TNFα simultaneous addition. (b). Inhibitors were added at -4 h 30 minutes, 2GF -4 h, and TNFα at time zero. 2GF were left on for the duration of the experiment (2GF cont) or left on for 15 minutes only (2GF pulse). RNA was isolated at three hours following TNFα stimulation. Data are normalized to levels with TNFα + vehicle. Asterisk indicates P < 0.05 to respective vehicle by ANOVA/Dunnett's (a) or ANOVA/Tukey-Kramer's (b) on log-transformed data.