Transcriptional regulation of matrix metalloproteinase-1 and collagen 1A2 explains the anti-fibrotic effect exerted by proteasome inhibition in human dermal fibroblasts

Table 1 Oligonucleotide sequences

Name	Sequence
Electrophoretic mobility shift assay
MMP-1 AP-1 S	CTAGTGATGAGTCAGCCGGATC
MMP-1 AP-1 AS	GATCCGGCTGACTCATCACTAG
Chromatin immunoprecipitation
MMP-1 AP-1 fw	CCTCTTGCTGCTCCAATATC
MMP-1 AP-1 rv	TCTGCTAGGAGTCACCATTTC
MMP-2 AP2 fw	GTGGAGGAGGGCGAGTAGGG
MMP-2 AP2 rv	CTGGGAGGGAGCTGGCAGAG
MMP-9 AP-1 fw	GAGAGGAGGAGGTGGTGTAAG
MMP-9 AP-1 rv	TTAAGGAGGCGCTCCTGTG
COL1A1 -200/+100 fw	CAGAGCTGCGAAGAGGGGA
COL1A1 -200/+100 rv	AGACTCTTTGTGGCTGGGGAG
COL1A2 fw2	GCGGAGGTATGCAGACAACG
COL1A2 rv1	GGGCTGGCTTCTTAAATTG
MMP-1 ORF fw	TAAGTACTGGGCTGTTCAGG
MMP-1 ORF rv	GAGCAGCATCGATATGCTTC
COL1A2 ORF fw	GCCCTCAAGGTTTCCAAG
COL1A2 ORF rv	GGGAGACCCATCATTTCAC
Reverse transcription-polymerase chain reaction
COL1A1	Hs00164004_m1
COL1A2	Hs00164099_m1
TIMP-1	Hs00171558_m1
MMP-1	Hs00233958_m1
MMP-2	Hs00234422_m1
HsEEF1A1	CACCTGAGCAGTGAAGCCAGCTGCTT
DNA pull-down assay
Biotin-MMP-1-S	GATCGAGAGGATGTTATAAAGCATGAGTCAG
Biotin-MMP-1-AS	CTGACTCATGCTTTATAACATCCTCTCGATC
Biotin-COL1A2-S	GAAAGGGCGGGGGAGGGCGGGAGGATGCGGAGGGCGGAG
Biotin-COL1A2-AS	CTCCGCCCTCCGCATCCTCCCGCCCTCCCCCGCCCTTTC

Bold case indicates proximal activation protein-1 (AP-1) site. Underlining indicates transcription binding sites (Ets and AP-1 for matrix metalloproteinase-1 [MMP-1] and SP1 for collagen 1A2 [COL1A2]).

ISSN: 1478-6362