Figure 2

Positive correlation of miR-146a expression with TNF-α levels in RA patients. (a) A positive correlation was found between miR-146a expression and TNF-α levels. Of synovial fluid (SF), 50% increased the expression of miR-146a in Jurkat T cells by 2.39 ± 0.34 fold and in human CD4⁺ T cells by 8.21 ± 1.19 fold (n = 4), respectively. (b) Induction of miR-146a expression stimulated with TNF-α or SF of rheumatoid arthritis (RA) in both Jurkat cells and CD4⁺ T cells. Unstimulated Jurkat T cells or CD4⁺ T cells were used as controls, respectively. The value of each control sample was set at one and further used to calculate the fold change. TNF-α upregulated miR-146a expression in Jurkat T cells and human CD4⁺ T cells in a dose-dependent fashion.