Figure 5

FAF1 abolishes the suppressive effect of miR-146a on Jurkat T cell apoptosis. (a) Fas-induced apoptosis was evaluated in Jurkat T cells transfected with either miR-146a alone or together with Fas-associated factor 1 (FAF1). miR-146a-overexpressing T cells showed significantly reduced apoptosis whereas co-overexpressing FAF1 abrogated the effect of miR-146a on T cell apoptosis. (b) After six hours of anti-Fas treatment, apoptotic Jurkat T cells were evaluated. The data are derived from three independent experiments (mean ± standard deviation). **P < 0.01.