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Figure 1 | Arthritis Research & Therapy

Figure 1

From: Clinical and serological evaluation of a novel CENP-A peptide based ELISA

Figure 1

Comparison between anti-CENP-A and anti-CENP-B reactivity. Correlation diagram is shown in a) Comparative receiver operating characteristic analysis for the discrimination between SSc patients (n = 131) and controls (n = 134) is shown in b). The area under the curve was significantly higher for CENP-A (0.81 vs. 0.47, P < 0.0001). Serum samples were selected that showed comparable results in CENP-A and CENP-B ELISA and diluted in Dilution Buffer to obtain a reactivity of approximately 1.0 OD. Increasing concentrations of CENP-A derived peptide and recombinant CENP-B were added to the diluted sera and incubated for two hours at room temperature. Specimens were then assayed in CENP-A and CENP-B ELISA. Inhibition was observed for anti-CENP-A reactivity with the CENP-A peptide c) and for anti-CENP-B reactivity with the recombinant CENP-B protein d), but not with the respective other antigen.

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