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Table 1 Qualitative agreement between CENP-A ELISA and other methods

From: Clinical and serological evaluation of a novel CENP-A peptide based ELISA

n = 99#, kappa = 0.73

IIF

 

CENP-A ELISA

pos (%)

neg (%)

Total

pos (%)

19 (19.2)

6 (6.1)

25 (25.3)

neg (%)

4 (4.0)

70 (70.7)

74 (74.8)

Total

23 (23.2)

76 (76.8)

99 (100.0)

n = 265*, kappa = 0.86§

CENP-A ELISA

 

CENP-B ELISA

pos (%)

neg (%)

Total

neg (%)

4 (1.5)

205 (77.4)

209 (78.9)

Total

52 (19.6)

213 (80.4)

265 (100.0)

n = 100#, kappa = 0.81§

LIA (CENP-B)

 

CENP-A ELISA

pos (%)

neg (%)

Total

pos (%)

20 (20.0)

5 (5.0)

25 (25.0)

neg (%)

2 (2.0)

73 (73.0)

75 (75.0)

Total

22 (22.0)

78 (78.0)

100 (100.0)

n = 100#, kappa = 0.88§

IIF

 

LIA CENP-B

pos (%)

neg (%)

Total

pos (%)

19 (19.0)

3 (3.0)

22 (22.0)

neg (%)

1 (1.0)

77 (77.0)

78 (78.0)

Total

20 (20.0)

80.0

100 (100.0)

n = 82#, kappa = 0.97§

EliA ® CENP-B

 

CENP-A ELISA

pos (%)

neg (%)

Total

pos (%)

32 (39.0)

1 (1.2)

33 (40.2)

neg (%)

0 (0.0)

49 (59.8)

78 (59.8)

Total

32 (39.0)

50 (61.0)

100 (100.0)

  1. * SSc patients and controls; # SSc patients only; §very good agreement
  2. CENP: centromere protein; ELISA: enzyme linked immunoassay; IIF: indirect immunofluorescence; LIA: line-immunoassay