Skip to main content

Advertisement

Table 1 Unstimulated fibroblast-like synoviocytes of patients with psoriatic arthritis or rheumatoid arthritis express higher levels of nerve growth factor and TrkA

From: Functional significance of nerve growth factor and its receptor (TrkA) in inflammatory arthritis

   FACS analyses, percentage of cells
Cells NGF, pg/mL NGF+ TrkA+ p75+
Unstimulated FLSs (meniscal injury n = 4) 30 ± 5 4.5 ± 0.3 1.33 ± 0.4 0.5 ± 0.05
FLSs + IL-1β (meniscal injury) 110 ± 28a 12.4 ± 2a 16.5 ± 5a 0.4 ± 0.2
FLSs + TNF-a (meniscal injury) 129 ± 23a 6.3 ± 4a 11.5 ± 5a 0.71 ± 0.4
FLSs from patients with PsA (n = 3) 105 ± 15a 17 ± 4a 10 ± 3a 1.0 ± 0.4
FLSs from patients with RA (n = 4) 65 ± 4b 8 ± 0.4b 5.5 ± 0.4b 0.5 ± 0.4
FLSs from patients with OA (n = 4) 34 ± 2 3.9 ± 0.2 0.9 ± 0.2 0.7 ± 0.2
  1. Third-passage fibroblast-like synoviocytes (FLSs) (5,000 cells in 200 μL of Dulbecco's modified Eagle's medium [DMEM] complete medium) in 96-well plates were cultured for 6 days. Unstimulated cells were cultured in DMEM only, and stimulated cells were cultured in DMEM with cytokines tumor necrosis factor-alpha (TNF-α) (1 ng/mL) and interleukin-1-beta (IL-1β) (1 ng/mL). Data are expressed as mean ± standard deviation. Nerve growth factor (NGF), TrkA, and p75 data of unstimulated FLSs were compared with NGF, TrkA, and p75 data of FLSs of other groups mentioned in the table. a/bSignificantly different from unstimulated cells (aP < 0.001, bP < 0.05 Student t test). FACS, fluorescence-activated cell sorting; OA, osteoarthritis; PsA, psoriatic arthritis; RA, rheumatoid arthritis.