Toll-like receptor 3 upregulation in macrophages participates in the initiation and maintenance of pristane-induced arthritis in rats

Table 1 Information of primers for conventional PCR and Real-time PCR

Gene name	NCBI Accession No.	Sequence(5'-3')		Size (bp)	annealing temperature (°C)
TLR1	NM_001172120	forward	CAGCAGCCTCAAGCATGTCTA
		reverse	CAGCCCTAAGACAACAATACAATAGAAGA	82	60
TLR2	NM_198769	forward	CTCCTGTGAACTCCTGTCCTT
		reverse	AGCTGTCTGGCCAGTCAAC	74	60
TLR3	NM_198791	forward	GATTGGCAAGTTATTCGTC
		reverse	GCGGAGGCTGTTGTAGG	205	54
TLR4	NM_019178	forward	GATTGCTCAGACATGGCAGTTTC
		reverse	CACTCGAGGTAGGTGTTTCTGCTAA	135	54
TLR5	XM_001063885	forward	GGGCAGCAGAAAGACGGTAT
		reverse	CAGGCACCAGCCATCCTTAA	61	60
TLR6	NM_207604	forward	AGAACCTTACTCATGTCCCAAAAGAC
		reverse	AGATCAGATATGGAGTTTTGAGACAGACT	79	60
TLR7	NM_001097582	forward	GTTTTACGTCTACACAGTAACTCTCTTCA
		reverse	TTCCTGGAGGTTGCTCATGTTTT	75	60
TLR8	NM_001101009	forward	GGGGTAACACACCGTCTA
		reverse	GTCAAGGCGATTTCCACT	150	60
TLR9	NM_198131	forward	CCGAAGACCTAGCCAACCT
		reverse	TGATCACAGCGACGGCAATT	70	60
TNF-α	NM_012675	forward	TCAGCCTCTTCTCATTCCTGC
		reverse	TTGGTGGTTTGCTACGACGTG	203	60
IL-6	NM_012589	forward	AAGAAAGACAAAGCCAGAGTC
		reverse	CACAAACTGATATGCTTAGGC	263	60
IFN-γ	NM_138880	forward	CCCTCTCTGGCTGTTACTGC
		reverse	TTTCGTGTTACCGTCCTTTTG	149	54
IFN-β	NM_019127	forward	CTTGGGTGACATCCACGACTAC
		reverse	GGCATAGCTGTTGTACTTCTTGTCTT	92	54
β-actin	NM_031144	forward	GAGGGAAATCGTGCGTGAC
		reverse	GCATCGGAACCGCTCATT	157	60
gfp	-	forward	GGCACCGATTTCAAGGAG
		reverse	CGCCGATGGGGGTATTCT	190	54

ISSN: 1478-6362