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Table 1 Thermal cycling conditions for real time PCR

From: Obesity affects the chondrocyte responsiveness to leptin in patients with osteoarthritis

Gene Sequences Temp (°C) Amplicon size (bp)
Aggrecan Fw TCG AGG ACA GCG AGG CC
Rv TCG AGG GTG TAG CGT GTA GAG A
61 85
Collagen type 2 Fw ATG ACA ATC TGG CTG CCA
Rv CTT CAG GGC AGT GTA CGT
58 200
IGF-1 Fw GTA TTG CGC ACC CCT CAA
Rv TTG TTT CCT GCA CTC CCT CT
57 126
TGFβ Fw TGC GGC AGC TGT ACA TTG A
Rv TGG TTG TAC AGG GCC AGG A
59 186
MMP-13 Fw TGG TGG TGA TGA AGA TGA TTT G
Rv TCT AAG CCG AAG AAA GAC TGC
57 125
TIMP-2 Fw CTT CTT TCC TCC AAC GTC
Rv AAA GCG GTC AGT GAG AAG GA
57 183
COX-2 Fw GCT GGA ACA TGG AAT TAC CCA
Rv CTT TCT GTA CTG CGG GTG GAA
58 98
iNOS Fw ACA AGC CTA CCC CTC CAG AT
Rv TCC CGT CAG TTG GTA GGT TC
59 157
IL-1 Fw GGA CAA GCT GAG GAA GAT GC
Rv TCG TTA TCC CAT GTG TCG AA
57 120
RP 29 Fw AAG ATG GGT CAC CAG CAG GTC TAC TG
Rv AGA CGC GGC AAG AGC GAG AA
59 153
  1. bp, base pairs; COX-2, cyclooxygenase-2; IGF-1, insulin growth factor-1; MMP, matrix metalloproteinase; iNOS, inducible nitric oxide synthase; Temp, temperature; TGF, transforming growth factor; TIMP, tissue inhibitor of metalloproteinase; IL-1, interleukin-1.