Skip to main content
Figure 2 | Arthritis Research & Therapy

Figure 2

From: Interleukin-18 as an in vivomediator of monocyte recruitment in rodent models of rheumatoid arthritis

Figure 2

IL-18 activates p-p38 and p-ERK½ in a time-dependent manner. Monocytes (5 × 106 cells) were stimulated with 2.5 nM rhuIL-18. Cell lysates were made and probed for p-p38 and p-ERK½ with Western blot, showing marked increases in phosphorylation after 5 minutes for p-p38 and 15 to 30 minutes for p-ERK½. Representative blots show both p-p38 and p-ERK½ (upper panel for p-p38 and lower panel for p-ERK½). Graphs for p-p38 and p-ERK½ were normalized by respective total cellular expression for both signaling molecules relative to the untreated control blots (n = the number of blood donors, and graphs show combined data from five separate experiments). In total, five separate experiments were completed by using peripheral blood monocytes from four separate volunteers.

Back to article page