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Figure 3 | Arthritis Research & Therapy

Figure 3

From: Interleukin-18 as an in vivomediator of monocyte recruitment in rodent models of rheumatoid arthritis

Figure 3

Monocytes were suspended at 2.5 × 106 cells/ml and then transfected with sense or antisense ODNs in serum-free media for 4 hours. Transfection efficiency for all genes was routinely > 80%, as determined by counting fluorescein isothiocyanate (FITC)-transfected cells with fluorescence microscopy and comparing with a DAPI label in the same cells (data not shown). Transfected cells were added to Boyden chemotaxis chambers to determine their migratory activity toward rhuIL-18 (2.5 nM). As shown, monocytes transfected with either antisense p38 or ERK½ showed significant reductions in chemotaxic activity toward rhuIL-18 compared with sense transfected cells (n = number of experimental repeats from independent PB monocyte donors).

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