Increased number of CD14+ cells in (PBMCs) from SSc patients. Peripheral blood mononuclear cells (PBMCs) isolated from healthy controls or systemic sclerosis (SSc) patients were analyzed by single-color flow cytometry for CD14 expression. Upper panel (a) shows the results of PBMCs from healthy controls (left), limited cutaneous systemic sclerosis (lcSSc) patients (middle), and diffuse cutaneous systemic sclerosis (dcSSc) patients (right). Values are the percentage of total PBMCs in each region. FL4, phycoerythrin-cyanin 5.1 fluorescence; SS, side scatter; #Cells = actual number of the cells. Data presented here are representative of 16 healthy controls and 41 SSc patients. Lower panel (b) depicts the summary of results, comparing percentages of CD14 positive cells in PBMCs (shown on the ordinate) from healthy controls and SSc patients. *P < 0.05 as compared with the value in cells from healthy controls.