Increased osteoarthritic changes in high-fat-fed high gainer mice. (a) Representative histological images of knee joints showing increased proteoglycan depletion in high gainer (HG) mice as indicated by a loss of the red safranin-O staining. Scale bar = 100 μm. (b) Representative histological images of temporomandibular joints in control, low gainer (LG) and HG mice. There is a nonsignificant trend (P = 0.10) for increased loss of safranin-O staining in LG mice and HG mice. Scale bar = 100 μm.