Central thermal hyperalgesia in high-fat-fed mice at 53 weeks of age. (a) Paw-withdrawal latency durations versus time for a hotplate test of centrally-mediated thermal hyperalgesia. High-fat (HF)-fed mice showed thermal hyperalgesia for the first 60 minutes of hotplate testing. (b) Tail-flick latency over time for a test of peripherally-mediated thermal hyperalgesia. HF-fed mice showed thermal hyperalgesia at 240 minutes of tail-flick testing. Data shown as mean ± standard error of the mean. *P < 0.05 for comparison with time-matched control value for either the low gainer (LG) group or the high gainer (HG) group. #P < 0.05 for time-matched HF diet versus control comparison.