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Figure 6 | Arthritis Research & Therapy

Figure 6

From: Innate immunity triggers IL-32 expression by fibroblast-like synoviocytes in rheumatoid arthritis

Figure 6

Effect of co-stimulation of either TLR ligands or TNF-α (10 ng/ml) and IFN-γ (0.1 ng/ml) on IRF-1 and IRF-3 mRNA expression. (a) Fibroblast-like synoviocytes (FLSs) were incubated simultaneously with IFN-γ and TNF-α, and IFN regular factor (IRF-1) mRNA expression was assessed after activation (4 and 24 h) by using quantitative RT-PCR. (b, c) Rheumatoid arthritis (RA) FLSs were transfected with IRF-1 antisense molecules or with a negative control (Control). Concomitant activation with IFN-γ and TNF-α of transfected RA FLSs was performed 24 h after transfection, for 4 h. IRF-1 (b) IL-32 (c) expression was determined with quantitative RT-PCR. (d) IL-6 release was determined with ELISA after co-stimulation with IFN-γ and TNF-α. (e) FLSs were incubated with IFN-γ or with IFN-γ and either LPS (1 μg/ml), BLP (1 μg/ml), or poly I:C (10 μg/ml) for 4 h. IFN regular factor (IRF-1) and IRF-3 mRNA expression was determined by using quantitative RT-PCR. (f) RA FLSs were transfected with IRF-1 antisense molecules or with a negative control (Control). Concomitant activation with IFN-γ and LPS of transfected RA FLSs was performed 24 h after transfection, for 4 h. IL-32 expression was determined with quantitative RT-PCR. Data are expressed as the mean ± SD of three experiments (triplicates) on samples from four different patients with RA. **P < 0.001.

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