Treatment with adenoX-rCB11-A9 can prevent CIA. (a) Groups of 10 DBA/1 mice were administered intra-articularly either adenoX-rCB11-A9 (square, circle) or adenoX-LacZ (triangle, diamond). The mice were immunized with type II collagen/complete Freund's adjuvant either three (square, triangle) or seven (circle, diamond) days after the injection and all mice were observed for the development of arthritis. The data points reflect the mean severity score (sum of the severity scores/total number of animals in the group) at each time point. As shown the adenoX-rCB11-A9 was extremely effective at preventing the development of arthritis, whether the mice were immunized three days prior to immunization (final severity scores 1.3 ± 1.5 vs 6.8 ± 5.3, P ≤ 0.025) or seven days prior to immunization (final severity scores 1.8 ± 2.0 vs 7.0 ± 2.5, P ≤ 0.005). The final incidence of arthritis was (square = 20%, triangle = 90%, P ≤ 0.003) and (circle = 20%, diamond= 100%, P ≤ 0.0004). (b) Photographs of an arthritic hind paw from a DBA/1 mouse injected with adenoX-lacZ (left panel) and a hind paw from a DBA/1 mouse injected with adenoX-rCB11-A9 (right panel).