Figure 2

CD4 + T cell CD25 and Foxp3 expression following incubation with pDCs or pDCs + apoPMNs in MLR systems. (a) Autologous MLR system T cell CD25 expression: No changes were detected on CD4 + T cells from either healthy subjects (n = 36) or patients with SLE (n = 30; 12 with active disease and 18 with inactive disease) following incubation with pDCs alone or pDCs + apoPMNs. Values are expressed in Median (interquartile range, range). (b) Allogenous MLR system T cell CD25 expression: CD25 expression was increased following incubation with healthy pDCs + apoPMNs (n = 36) but not pDCs alone, whereas a significant increase was seen following incubation with both SLE pDCs alone and pDCs + apoPMNs (n = 30). Values are expressed in Median (interquartile range, range). (c) Autologous MLR system CD4 + CD25 + T cell intracellular staining of Foxp3: No changes were detected following incubation with either pDCs alone or pDCs + poPMNs from healthy subjects (n = 6) and patients with SLE (n = 6). Values are expressed in Mean ± SEM. (d) Allogenous MLR system CD4 + CD25 + T cell intracellular staining of Foxp3: pDCs + apoPMNs from healthy donors increased the expression of Foxp3 on allogenous CD4 + CD25 + T cells (n = 8, P < 0.05), but no significant changes were seen with SLE pDCs alone or pDCs + apoPMNs (n = 8). Values are expressed in Mean ± SEM. auto-T: autologous T cells, allo-T: allogenous T cells, pDCs + apoPMNs: pDCs that had been loaded with apoptotic polymorphonuclear cells. HD, healthy donors; aSLE, active SLE; iSLE, inactive SLE.