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Table 1 Human articular chondrocyte proteins modified by treatment with interleukin-1β (IL-1β) plus glucosamine and/or chondroitin sulfate

From: Pharmacoproteomic study of the effects of chondroitin and glucosamine sulfate on human articular chondrocytes

Spot n°

 

Protein name

Acc. n°§

GS‡

CS‡

GS+CS‡

Loc.**

Mr/pI§§

Cellular role

1

PDIA1

Protein disulfide-isomerase precursor

P07237

6.54

5.60

11.24

ER, CM

57.1/4.76

Protein folding

2

ANXA5

Annexin A5

P08758

1.97

1.30

1.52

C

35.9/4.94

Signal transduction

3

GDIR

Rho GDP-dissociation inhibitor 1

P52565

2.62

1.03

2.51

C

23.2/5.03

Signal transduction

4

GRP78

78 kDa glucose-regulated protein precursor

P11021

8.08

1.19

14.15

ER

72.3/5.07

Protein folding

5

CO6A1

Collagen alpha-1(VI) chain precursor

P12109

4.14

-1.96

-1.5

EXC

108.5/5.26

Cell adhesion

6

ACTB

Actin, cytoplasmic 1

P60709

-3.7

-1.41

-1.89

C, CK

41.7/5.29

Cell motion

7

HSP7C

Heat shock cognate 71 kDa protein

P11142

7.20

3.90

5.46

C

70.9/5.37

Protein folding

8

GSTP1

Glutathione S-transferase P

P09211

-1.2

-1.54

-1.49

C

23.3/5.43

Detoxification

9

HSPB1

Heat shock protein beta-1

P04792

-1.33

-1.35

-1.75

C, N

22.8/5.98

Stress response

10

PDIA3

Protein disulfide-isomerase A3 precursor

P30101

9.59

9.74

12.50

ER

56.8/5.98

Protein folding

11

PDIA3

Protein disulfide-isomerase A3 precursor

P30101

10.24

5.29

7.13

ER

56.8/5.98

Protein folding

12

GELS

Gelsolin

P06396

5.93

3.12

3.98

C, CK

85.7/5.90

Actin depolymerizer

13

HSPB1

Heat shock protein beta-1

P04792

1.94

-1.25

1.26

C, N

22.8/5.98

Stress response

14

GANAB

Neutral alpha-glucosidase AB

Q14697

1.15

-1.56

-1.09

ER, G

106.9/5.74

CH Metabolism

15

ANXA1

Annexin A1

P04083

1.56

1.72

1.90

C, N, CM

38.7/6.57

Signal transduction

16

SEPT2

Septin-2

Q15019

1.08

-1.51

-1.35

N

41.5/6.15

Cell cycle/division

17

ENOA

Alpha-enolase

P06733

1.04

1.91

1.89

C, CM

47.2/7.01

Glycolysis

18

EF1G

Elongation factor 1-gamma

P26641

-1.28

-1.85

-1.92

C

50.2/6.25

Protein synthesis

19

TCPG

T-complex protein 1 subunit gamma

P49368

-1.39

-1.54

-1.96

C

60.5/6.10

Protein folding

20

DPYL2

Dihydropyrimidinase-related protein 2

Q16555

-1.12

-1.45

-1.79

C

62.3/5.95

Metabolism

21

SODM

Superoxide dismutase mitochondrial

P04179

-2.5

-1.3

-4.35

MIT

24.7/8.35

Redox

22

PGAM1

Phosphoglycerate mutase 1

P18669

-1.33

-1.23

-1.54

C

28.8/6.67

Glycolysis

23

TPIS

Triosephosphate isomerase

P60174

-1.69

-1.49*

-1.72

C

26.7/6.45

Glycolysis

24

ANXA2

Annexin A2

P07355

3.44

6.80

5.12

EXC, CM

38.6/7.57

Trafficking

25

AK1C2

Aldo-keto reductase family 1 member C2

P52895

-2

-2.13

-3.22

C

36.7/7.13

Metabolism

26

ENOA

Alpha-enolase

P06733

-1.96

-1.37

-1.92

C, CM

47.2/7.01

Glycolysis

27

UGDH

UDP-glucose 6-dehydrogenase

O60701

-1.16

-2.08

-1.85

C

55.0/6.73

Metabolism

28

ANXA2

Annexin A2

P07355

2.69

3.06

2.82

EXC, CM

38.6/7.57

Trafficking

29

PGK1

Phosphoglycerate kinase 1

P00558

-1.14

-2.33

-2.32

C

44.6/8.30

Glycolysis

30

ATPA

ATP synthase subunit alpha, mitochondrial

P25705

-1.43

-2.17

-2.22

MIT

59.8/9.16

Respiration

31

KPYM

Pyruvate kinase isozymes M1/M2

P14618

-1.59

-2.44

-2.5

C

57.9/7.96

Glycolysis

32

TAGL2

Transgelin-2

P37802

1.31

-1.09

-1.43*

CK, CM

22.4/8.41

Structural

33

PRDX1

Peroxiredoxin-1

Q06830

1.67

-1.12

-1.09

C

22.1/8.27

Redox

34

G3P

Glyceraldehyde-3-phosphate dehydrogenase

P04406

-1.27

-2.04

-2.63

C, CM

36.1/8.57

Glycolysis

35

ALDOA

Fructose-bisphosphate aldolase A

P04075

-1.22

-1.79

-1.89

C

39.4/8.30

Glycolysis

  1. §Protein accession number according to SwissProt and TrEMBL databases.
  2. ‡Average volume ratio vs IL-1β, quantified by PDQuest 7.3.1. software. * Protein altered less than 1.5-fold but with a significance level above 95% by the Student's t-test (p< 0.05).
  3. ** Predicted subcellular localization according to PSORTII program.
  4. §§Theoretical molecular weight (Mr) and isoelectric point (pI) according to protein sequence and Swiss-2DPAGE database.
  5. MIT, mitochondria; ER, endoplasmic reticulum; C, cytoplasm; CM, cell membrane; CK, cytoskeleton; G, Golgi apparatus; N, nucleus; EXC, extracellular matrix.
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Arthritis Research & Therapy

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