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Table 1 Human articular chondrocyte proteins modified by treatment with interleukin-1β (IL-1β) plus glucosamine and/or chondroitin sulfate

From: Pharmacoproteomic study of the effects of chondroitin and glucosamine sulfate on human articular chondrocytes

Spot n°   Protein name Acc. n°§ GS CS GS+CS Loc.** Mr/pI§§ Cellular role
1 PDIA1 Protein disulfide-isomerase precursor P07237 6.54 5.60 11.24 ER, CM 57.1/4.76 Protein folding
2 ANXA5 Annexin A5 P08758 1.97 1.30 1.52 C 35.9/4.94 Signal transduction
3 GDIR Rho GDP-dissociation inhibitor 1 P52565 2.62 1.03 2.51 C 23.2/5.03 Signal transduction
4 GRP78 78 kDa glucose-regulated protein precursor P11021 8.08 1.19 14.15 ER 72.3/5.07 Protein folding
5 CO6A1 Collagen alpha-1(VI) chain precursor P12109 4.14 -1.96 -1.5 EXC 108.5/5.26 Cell adhesion
6 ACTB Actin, cytoplasmic 1 P60709 -3.7 -1.41 -1.89 C, CK 41.7/5.29 Cell motion
7 HSP7C Heat shock cognate 71 kDa protein P11142 7.20 3.90 5.46 C 70.9/5.37 Protein folding
8 GSTP1 Glutathione S-transferase P P09211 -1.2 -1.54 -1.49 C 23.3/5.43 Detoxification
9 HSPB1 Heat shock protein beta-1 P04792 -1.33 -1.35 -1.75 C, N 22.8/5.98 Stress response
10 PDIA3 Protein disulfide-isomerase A3 precursor P30101 9.59 9.74 12.50 ER 56.8/5.98 Protein folding
11 PDIA3 Protein disulfide-isomerase A3 precursor P30101 10.24 5.29 7.13 ER 56.8/5.98 Protein folding
12 GELS Gelsolin P06396 5.93 3.12 3.98 C, CK 85.7/5.90 Actin depolymerizer
13 HSPB1 Heat shock protein beta-1 P04792 1.94 -1.25 1.26 C, N 22.8/5.98 Stress response
14 GANAB Neutral alpha-glucosidase AB Q14697 1.15 -1.56 -1.09 ER, G 106.9/5.74 CH Metabolism
15 ANXA1 Annexin A1 P04083 1.56 1.72 1.90 C, N, CM 38.7/6.57 Signal transduction
16 SEPT2 Septin-2 Q15019 1.08 -1.51 -1.35 N 41.5/6.15 Cell cycle/division
17 ENOA Alpha-enolase P06733 1.04 1.91 1.89 C, CM 47.2/7.01 Glycolysis
18 EF1G Elongation factor 1-gamma P26641 -1.28 -1.85 -1.92 C 50.2/6.25 Protein synthesis
19 TCPG T-complex protein 1 subunit gamma P49368 -1.39 -1.54 -1.96 C 60.5/6.10 Protein folding
20 DPYL2 Dihydropyrimidinase-related protein 2 Q16555 -1.12 -1.45 -1.79 C 62.3/5.95 Metabolism
21 SODM Superoxide dismutase mitochondrial P04179 -2.5 -1.3 -4.35 MIT 24.7/8.35 Redox
22 PGAM1 Phosphoglycerate mutase 1 P18669 -1.33 -1.23 -1.54 C 28.8/6.67 Glycolysis
23 TPIS Triosephosphate isomerase P60174 -1.69 -1.49* -1.72 C 26.7/6.45 Glycolysis
24 ANXA2 Annexin A2 P07355 3.44 6.80 5.12 EXC, CM 38.6/7.57 Trafficking
25 AK1C2 Aldo-keto reductase family 1 member C2 P52895 -2 -2.13 -3.22 C 36.7/7.13 Metabolism
26 ENOA Alpha-enolase P06733 -1.96 -1.37 -1.92 C, CM 47.2/7.01 Glycolysis
27 UGDH UDP-glucose 6-dehydrogenase O60701 -1.16 -2.08 -1.85 C 55.0/6.73 Metabolism
28 ANXA2 Annexin A2 P07355 2.69 3.06 2.82 EXC, CM 38.6/7.57 Trafficking
29 PGK1 Phosphoglycerate kinase 1 P00558 -1.14 -2.33 -2.32 C 44.6/8.30 Glycolysis
30 ATPA ATP synthase subunit alpha, mitochondrial P25705 -1.43 -2.17 -2.22 MIT 59.8/9.16 Respiration
31 KPYM Pyruvate kinase isozymes M1/M2 P14618 -1.59 -2.44 -2.5 C 57.9/7.96 Glycolysis
32 TAGL2 Transgelin-2 P37802 1.31 -1.09 -1.43* CK, CM 22.4/8.41 Structural
33 PRDX1 Peroxiredoxin-1 Q06830 1.67 -1.12 -1.09 C 22.1/8.27 Redox
34 G3P Glyceraldehyde-3-phosphate dehydrogenase P04406 -1.27 -2.04 -2.63 C, CM 36.1/8.57 Glycolysis
35 ALDOA Fructose-bisphosphate aldolase A P04075 -1.22 -1.79 -1.89 C 39.4/8.30 Glycolysis
  1. §Protein accession number according to SwissProt and TrEMBL databases.
  2. Average volume ratio vs IL-1β, quantified by PDQuest 7.3.1. software. * Protein altered less than 1.5-fold but with a significance level above 95% by the Student's t-test (p< 0.05).
  3. ** Predicted subcellular localization according to PSORTII program.
  4. §§Theoretical molecular weight (Mr) and isoelectric point (pI) according to protein sequence and Swiss-2DPAGE database.
  5. MIT, mitochondria; ER, endoplasmic reticulum; C, cytoplasm; CM, cell membrane; CK, cytoskeleton; G, Golgi apparatus; N, nucleus; EXC, extracellular matrix.