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Figure 1 | Arthritis Research & Therapy

Figure 1

From: Visualization and phenotyping of proinflammatory antigen-specific T cells during collagen-induced arthritis in a mouse with a fixed collagen type II-specific transgenic T-cell receptor β-chain

Figure 1

B22a1 binding requires a combination of Vβ12 and Vα16 T-cell receptor chain. (a) Flow cytometric analysis of T-cell hybridoma expressing different Vα-chain and Vβ-chain combinations stained with the biotinylated antibody B22a1 and allophycocyanin-conjugated streptavidin. Histograms show the staining intensities of negative control (FMO; stained with allophycocyanin-conjugated streptavidin only), B22a1 (straight line) for specificity and T-cell receptor (TCR) cβ (dotted line) for TCR surface expression levels. (b) For stimulation, T-cell hybridomas were cultured for 24 hours in precoated cell culture plates with titrated amounts of B22a1, TCR cβ (positive control) and CD4 (negative control), whereupon IL-2 production was determined by ELISA. Ab, antibody; FU, fluorescence units.

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Arthritis Research & Therapy

ISSN: 1478-6362

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