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Figure 2 | Arthritis Research & Therapy

Figure 2

From: Visualization and phenotyping of proinflammatory antigen-specific T cells during collagen-induced arthritis in a mouse with a fixed collagen type II-specific transgenic T-cell receptor β-chain

Figure 2

B22a1+ T cells dominate the collagen type II-specific T-cell expansion in Vβ12 transgenic mice. Lymph nodes of naïve and collagen type II (CII)-primed mice 10 days post immunization were restimulated for 6 hours in the presence of GalHyK264 and Brefeldin A. Flow cytometry was subsequently performed to determine B22a1 frequencies and CD40L upregulation. (a) Dot plots show B22a1 mean frequency among CD4 cells in Vβ12-transgenic (Vβ12-tg) mice (n = 5) and wildtype (WT) littermates (n = 4 and n = 5) left untreated (naïve) or immunized 10 days earlier. (b) Left panel: percentage of CD4 T cells upregulating CD40L upon restimluation with GalHyK264 in immunized Vβ12-transgenic mice and WT littermates. Insert: frequency of CD40L upregulation following stimulation with the nonmodified K264-peptide. Middle panel: distribution of B22a1 staining among CD4+CD40L+ T cells. Right panel: quadrant gates of CD4-gated T cells. Numbers indicate frequencies (mean ± standard error of the mean) of two individual mice per group, reproduced in at least two independent experiments (b).

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