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Figure 5 | Arthritis Research & Therapy

Figure 5

From: Visualization and phenotyping of proinflammatory antigen-specific T cells during collagen-induced arthritis in a mouse with a fixed collagen type II-specific transgenic T-cell receptor β-chain

Figure 5

B22a1+ T cells dominate during arthritis and are the main producers of proinflammatory cytokines. Vβ12-transgenic mice were primed with collagen type II (CII) and lymphocytes were analyzed 10, 25 and 35 days after immunization, following 6 hours of restimulation in the presence of the GalHyK264 peptide and Brefeldin A. (a) Representative dot plot from day 35 analysis and a summarizing bar graph showing the frequencies of CD4+ T cells being B22a1-and B22a1+ and expressing CD40L. (b) Cytokine production of activated CII-specific T cells, determined by gating on total CD40L+CD4+ T cells. Bars represent mean percentage (± standard error of the mean) of cytokine-producing cells among CD4+CD40L+ T cells, stratified according to B22a1 expression. IL-2 bars show frequency of cells producing IL-2 only, whereas IFNγ and IL-17 bars include IFNγ and IL17 single positives as well as IFNγ/IL-2 and IL-17/IL-2 double positives, respectively. The IFNγ and IL-17 bars are not adjusted for IFNγ/IL-17 double-positive cells due to their low abundance. All plots include 12 mice, eight mice and five mice for day 10, day 25 and day 35, respectively. *P < 0.05, **P < 0.01, ***P < 0.001.

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