Visualization and phenotyping of proinflammatory antigen-specific T cells during collagen-induced arthritis in a mouse with a fixed collagen type II-specific transgenic T-cell receptor β-chain

Table 1 Binding specificities of the B22a1 antibody towards different T-cell receptors

Clone	TCR Vα chain	TCR Vβ chain	Antigen specificity	SI	TCR cβ geometric mean	SI normalized to TCR^a
22a1-7E	16	12	GalHyK264	24.0	110	218.1
18b4-10D	16	12	GalHyK264	12.3	431	28.6
3D4-1.2	16	12	GalHyK264 and GlcGalHyK264	1.1	431	2.5
HCQ.3	16	8.1	GalHyK264	0.1	357	0.2
HM1R.2	4	12	GalHyK264	0.9	505	1.7
HCQ.4	4	12	K264	0.1	316	0.3
HRC.2	2	20	K264	0.3	591	0.5

Table showing the staining index (SI) of B22a1 and the SI normalized to the T-cell receptor (TCR) expression, respectively. GalHyK264, CII(260-270) with a β-D-galactopyranosyl residue on L-hydroxylysine at position 264; GlcGalHyK264, CII(260-270) with an α-D-glucopyranosyl-(1 > 2)-β-D-galactopyranosyl residue on L-hydroxylysine at position 264; K264, CII(260-270) with a nonmodified lysine at position 264; TCR cβ, anti-TCR cβ antibody (clone H57-597). ^a SI normalized to TCR is multiplied by 1,000.

ISSN: 1478-6362