Figure 1
From: Approaches to enhancing the retroviral transduction of human synoviocytes
IL-1Ra production by human synoviocytes after transduction by MFG-IRAP under various conditions. Monolayer cultures of synoviocytes from five different patients with RA were transduced with standard-titer MFG-IRAP under the following conditions: A) first passage cells; static conditions; single transduction; B) second passage cells; static conditions; single transduction; C) first passage cells; with centrifuging; single transduction; D) first passage cells; static conditions; double transduction. Each point represents the average of two determinations for each patient under the indicated conditions. To the left of each column are the overall means for each method ± SD. The dashed line indicates the minimum level of 30 ng IL-1Ra/106 cells per 48 h required by the clinical protocol. IL-1Ra production after transduction with centrifuging (column C) exceeds all other methods, with P < 0.0001.