A new concept for Urate in human serum: Evidences that support the existence of Urate as loosely associated and protein-bound Urate in human serum

Table 1 The absorption spectra of both the diluted serum sample and the pure uric acid solution before and after the action of uricase

The serum sample: A : The absorbance before the addition of uricase. B : The absorbance after 2 hours incubation with uricase. A-B : The difference between absorbances before and after incubation for 2 hours with uricase, the max. decrease is at 283–285 nm. C : The absorbance after 48 hours incubation with uricase, the abosrbance recorded was higher than that recorded before the action of uricase over the whole region of measurement (270–300 nm). The pure uric acid solution: U₀ : The absorbance before the addition of uricase. U₂ : The absorbance two hours after the action of uricase. U₀ - U₂ : The difference between absorbance before and after two hours incubation with uricase, the max. decrease is at 293 nm.

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