A new concept for Urate in human serum: Evidences that support the existence of Urate as loosely associated and protein-bound Urate in human serum

Table 2 The absorption spectra of both the precipitated protein solution and the protein-free supernatant before and after the action of uricase

The precipitated-protein soution: B₀ : The asorbance before the addition of uricase. B₂₄ : The absorbance after 24 hours incubation with uricase. B₀-B₂₄ : The decrease in the absorbance after incubation for 24 hours with uricase, the max. decrease is at 283 nm. B₇₂ : The absorbance after 72 hours incubation with uricase, the absorbance recorded was higher than that recorded before the action of uricase over the whole region of measurement (270-300 nm). The protein -free supernatant : A₀ : The absorbance before the addition of uricase. A₂ : The absorbance two hours after the action of uricase. A₀ - A₂ : The difference between absorbance before and after two hours incubation with uricase, the max. decrease reached at 293 nm.

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