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Figure 4 | Arthritis Research & Therapy

Figure 4

From: Monocytes/macrophages express chemokine receptor CCR9 in rheumatoid arthritis and CCL25 stimulates their differentiation

Figure 4

CCL25 expression in the rheumatoid synovium. Sections were treated with antibodies to CCL25 and CD68, followed by double label immunofluorescence microscopy. (a) Shows staining for CD68 (red) in cells of the synovial sublining. (b) Same field of view as A labelled for CCL25 (green). (c) Shows staining for nuclei using DAPI (blue). (d) Merge of A, B and C showing CD68+CCL25+ cells. Arrows indicate examples of cells where CD68 and CCL25 colocalise. The bar represents 100 μm. E, CCL25 mRNA in synovial tissue by RT-PCR. RNA isolated from the synovia of rheumatoid arthritis patients (n = 8) and controls (n = 7) showing the presence of CCL25 mRNA. L27 was used for normalisation.

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