Figure 2

Confocal analysis of HMGB1 expression in human synovium. Representative immunohistochemical sections of human (a, c) normal (n = 3) and (b, d) osteoarthritic (n = 3) synovium with high mobility group box 1 (HMGB1) revealed in fluorescence and read with confocal microscopy. (e) represents immunohistochemistry revealed in 3,3'-diaminobenzidine (DAB) of human osteoarthritic synovium infiltrates, and (f) is as (e) but revealed with the fluorescent antibody. The colour green represents HMGB1 and blue the nucleus. Original magnification × 100. (c) and (d) were amplified by the image processor software.