Skip to main content
Figure 3 | Arthritis Research & Therapy

Figure 3

From: High mobility group box 1 potentiates the pro-inflammatory effects of interleukin-1β in osteoarthritic synoviocytes

Figure 3

Effect of HMGB1 and IL-1β on the levels of cytokine and chemokine released into the medium by osteoarthritic synoviocytes. (a) IL-6, (b) IL-8, (c) CCL2 and (d) CCL20 protein levels. Cells were stimulated with IL-1b (10 ng/ml) for 24 hours in the presence or absence of high mobility group box 1 (HMGB1) at 15 and 25 ng/ml. Protein levels were determined in supernatants by ELISA. Data are expressed as mean ± standard error of the mean. Duplicate samples from six patients were used. ++P < 0.01 with respect to nonstimulated cells. *P < 0.05, **P < 0.01 with respect to IL-1β.

Back to article page