Figure 4

Effect of HMGB1 and IL-1β on cytokine and chemokine mRNA levels in OA synoviocytes. (a) IL-6, IL-8, and (b) CCL2 and CCL20 mRNA relative expression. Cells were stimulated with IL-1b (10 ng/ml) for 24 hours in the presence or absence of high mobility group box 1 (HMGB1) at 15 and 25 ng/ml. mRNA expression was determined by real-time PCR. Data are expressed as mean ± standard error of the mean. Duplicate samples from four patients were used. ++P < 0.01 with respect to nonstimulated cells. *P < 0.05 with respect to IL-1β.