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Figure 7 | Arthritis Research & Therapy

Figure 7

From: High mobility group box 1 potentiates the pro-inflammatory effects of interleukin-1β in osteoarthritic synoviocytes

Figure 7

Effect of HMGB1 and IL-1β on Akt and MAPK phosphorylation. Cells were stimulated with IL-1b (10 ng/ml) for five minutes in the presence or absence of high mobility group box 1 (HMGB1) at 15 and 25 ng/ml. Protein level was determined in cell lysates by western blotting by using specific antibodies against phosphorylated or total proteins. Relative expression of phosphorylated and total protein bands was calculated after densitometric analysis. Data are expressed as mean ± standard error of the mean (samples from three patients). ++P < 0.01 with respect to nonstimulated cells. *P < 0.05, with respect to IL-1β. ERK, extracellular signal-regulated kinase; JNK, c-Jun N-terminal kinase; MAPK, mitogen-activated protein kinase.

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