Figure 8

Effect of HMGB1 and IL-1β on NF-κB activation. Transient transfection was performed with the reporter construct nuclear factor (NF)-κB-luc and the internal control pRL-TK, as indicated in Materials and Methods. Cells were treated for 24 hours with high mobility group box 1 (HMGB1) at 25 ng/ml in the absence or presence of IL-1β (10 ng/ml). Firefly luciferase activity was normalized to Renilla luciferase activity. Data are expressed as mean ± standard error of the mean. Duplicate samples from six patients were used. ++P < 0.01 with respect to nonstimulated cells; *P < 0.05 with respect to IL-1β.