Resveratrol suppressed AGEs-induced MMP-13 activation and prevented AGEs-mediated collagen II destruction. Chondrocytes were pretreated with various doses of resveratrol for 24 h and then stimulated with 100 μg/ml AGEs for another 24 h. The activities of MMP-13 released into the culture supernatants were determined by gelatin zymography as described in Materials and Methods (a). The results from three independent experiments were shown (b). In (c), after treatment, the total cellular RNA was prepared and real time RT/PCR was applied to measure the levels of MMP-13 mRNA. In (d), chondrocytes were pretreated with various doses of resveratrol for 24 h, stimulated with 100 μg/ml AGEs for another 24 h and then the levels of collagen II in total cell lysates were determined by Western blot. *: P < 0.05 compared to the AGEs-stimulated in the absence of resveratrol treatment.