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Table 2a Recoveries of uric acid from serum Uric acid was added to the serum before incubation with the enzymatic color reagent, P.B.U didn't include in the reaction

From: A new concept for urate in human serum: Enzymatic assay of total urate (protein-bound & loosely associated) in serum using 3,5-dichloro-2-hydroxybenzenesulphonic acid/4-aminophena zone chromogenic system

Initial urate

Uric acid

Uric acid

Recovery %

present

added

found

   

Loosely

Total

 

After 15

At the end of

Loosely

P.B.U

Total

associated

urate

 

min

48 h

associated

 

recovery

   

incubation

incubation

   

7.6

27.7

2

9.6

9.5

100

-

31.98

3.4

14.2

5

8.3

8.4

98.8

-1.2

43.75

3.4

14.2

10

13.3

11.1

99.3

-0.70

45.8