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Figure 4 | Arthritis Research & Therapy

Figure 4

From: Development of a macromolecular prodrug for the treatment of inflammatory arthritis: mechanisms involved in arthrotropism and sustained therapeutic efficacy

Figure 4

Immunohistochemical staining of sections from inflamed joints with anti-CD68 or anti-prolyl-4-hydroxylase antibodies. Representative confocal microscopic images of (a) anti-CD68 and (b) anti-prolyl-4-hydroxylase immunohistochemical staining of decalcified, Alexa Fluor® 488-labeled N-(2-hydroxypropyl)methacrylamide copolymer (P-Alexa)-treated adjuvant-induced arthritis (AA) rat ankle joint sections are summarized in this figure. Each panel is composed of four sub-images: antibody red staining, P-Alexa green signal, differential interference contrast (DIC) image and the co-localization of the three. The co-localization of red and green color in both panels yields a yellow color, which confirms the uptake of the HPMA copolymer conjugate by CD68-positive (macrophage-like) or prolyl-4-hydroxylase-positive (fibroblast-like) synoviocytes in AA rat ankle joints.

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