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Table 1b b) U.V. study : The absorption spectra of : (i) diluted serum. (ii) just of the addition of the 0.4 mol/L sod. hydroxide solution. (iii) After 30 minutes incubation with the 0.4 mol/L sodium hydroxide solution.

From: A new concept for urate in human serum: Colorimetric assay of total urate-equivalent chromogens in human serum

W.L

Absorbance

 

(i)

(ii)

(iii)

270

0.32

0.500

0.55

275

0.36

0.470

0.58

280

0.42

0.440

0.68*

283

0.43*

0.520

0.67

285

0.42

0.520

0.65

290

0.34

0.510

0.55

293

0.30

0.525*

0.52

295

0.25

0.520

0.52

300

0.17

0.515

0.52

  1. (i) Shows max. absorbance at 283 nm. (ii) Shows max. absorbance at 293 nm. (iii) Shows max. absorbance at 280 nm.
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Arthritis Research & Therapy

ISSN: 1478-6362

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