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Table 1b b) U.V. study : The absorption spectra of : (i) diluted serum. (ii) just of the addition of the 0.4 mol/L sod. hydroxide solution. (iii) After 30 minutes incubation with the 0.4 mol/L sodium hydroxide solution.

From: A new concept for urate in human serum: Colorimetric assay of total urate-equivalent chromogens in human serum

W.L Absorbance
  (i) (ii) (iii)
270 0.32 0.500 0.55
275 0.36 0.470 0.58
280 0.42 0.440 0.68*
283 0.43* 0.520 0.67
285 0.42 0.520 0.65
290 0.34 0.510 0.55
293 0.30 0.525* 0.52
295 0.25 0.520 0.52
300 0.17 0.515 0.52
  1. (i) Shows max. absorbance at 283 nm. (ii) Shows max. absorbance at 293 nm. (iii) Shows max. absorbance at 280 nm.