Figure 4

μCT overlays visualzing bone changes in proximal tibia from start to end of experiment. Overlaid registered longitudinal cross-section of proximal tibia scanned at start (week 0) and end of experiment (week 12) for (a) OVX+Sal mouse, (b) OVX+IA mouse, and (c) OVX+E+IA mouse. M indicates medial, L indicates lateral. Dark grey: present at both time points; black: only present at week 0 (i.e., resorbed in 12 weeks); light grey: only present at week 12 (i.e., newly formed in 12 weeks). Note the thinning of the subchondral plate (open arrowheads in (b)), loss of trabeculae in the metaphysis (both (a) and (b)), and the osteophyte formation at the medial epiphysis (closed arrowhead in (b) and (c)).