Figure 3

Gene expression of p38-MAPK isoforms in primary human OA chondrocytes. (a) Total RNA was prepared from the human OA chondrocytes, single stranded cDNA was synthesized and the PCR reaction was carried out using specific primers for p38-MAPKα, β, γ and δ isoforms as described in methods section above. (b) Protein expression of p38-MAPK isoforms in human OA chondrocytes, C28-I2 human chondrocytes and Hela cells. Western blot analysis was performed as described under Figure 1. Band images were digitally captured and the band intensities (pixels/band) were obtained using the Un-Scan-It software. Data shown are cumulative of two experiments. Average pixel values presented as Mean ± SD; data without a common letter differ, P < 0.05.