Figure 5

u-PA^-/- mice show sustained synovial gene expression of inflammatory and destructive mediators during antigen-induced arthritis. Relative mRNA gene expression in the synovial tissue from u-PA^-/- and C57BL/6 mice at 1, 2, and 6 weeks after intra-articular injection in the antigen-induced arthritis model. (a) Interleukin-1-beta (IL-1β). (b) Tumor necrosis factor (TNF). (c) ADAMTS-4. (d) ADAMTS-5. (e) Matrix metalloproteinase-3 (MMP-3). (f) MMP-13. Results are expressed as the mean ± standard error of the mean (five mice per group). *P < 0.01, C57BL/6 versus u-PA^-/- mice; P < 0.05, C57BL/6 IL-1β, TNF, MMP-13 over time, P = 0.01, C57BL/6 ADAMTS-4, MMP-3 over time; P < 0.01, u-PA^-/- MMP-13 over time. IL-1β, ADAMTS-5, and MMP-13 were not detectable in C57BL/6 mice at week 6. ADAMTS, a disintegrin and metalloproteinase with thrombospondin motifs; u-PA^-/-, urokinase-type plasminogen activator gene-deficient.