Intradiscal transplantation of synovial mesenchymal stem cells prevents intervertebral disc degeneration through suppression of matrix metalloproteinase-related genes in nucleus pulposus cells in rabbits

Table 1 Rat genes up- and down- regulated two-fold or higher in nucleus pulposus cells co-cultured with human synovial MSCs

Genes	Genbank	Fold change
Collagens
Col2a1	[Genbank:AF305418]	10.5
Col1a1	[Genbank:Z78279]	-2.3
Col5a1	[Genbank:NM_134452]	-7.1
Col5a3	[Genbank:NM_021760]	-25.3
Proteoglycans
Chondroitin sulfate proteoglycan 2	[Genbank:AF084544]	2.9
Biglycan	[Genbank:NM_017087]	-7.6
Glypican 1	[Genbank:NM_030828]	-13.4
Lumican	[Genbank:NM_031050]	-16.1
Chondroitin sulfate proteoglycan 4	[Genbank:NM_031022]	-18.1
Tissue inhibitor of metalloproteinases
TIMP-3	[Genbank:NM_012886]	27.3
TIMP-1	[Genbank:NM_053819]	-2.1
TIMP-2	[Genbank:NM_021989]	-4.7
Matrix metalloproteinases
MMP-23	[Genbank:NM_053606]	-2.0
MMP-16	[Genbank:NM_080776]	-3.0
MMP-11	[Genbank:NM_012980]	-3.7
MMP-14	[Genbank:X83537]	-6.0
MMP-3	[Genbank:NM_133523]	-44.5
MMP-13	[Genbank:M60616]	-59.7
MMP-2	[Genbank:U65656]	-82.7
Interleukin related
Nuclear factor, IL-3 regulated	[Genbank:NM_053727]	-2.3
IL-15	[Genbank:AF015718]	-3.5
IL-6 signal transducer	[Genbank:AI171807]	-7.4
IL-11 receptor, alpha chain 1	[Genbank:AF347936]	-9.3
Tumor necrosis factor related
TNF receptor superfamily, member 1a (TNFRSF1A)	[Genbank:NM_013091]	-2.0
TNF-α converting enzyme	[Genbank:NM_020306]	-2.6
TNF-α induced protein 6 (TSG-6)	[Genbank:AF159103]	-5.4
Type 1 TNF receptor shedding aminopeptidase regulator	[Genbank:NM_030836]	-9.0
TNF receptor superfamily, member 6 (TNFRSF6)	[Genbank:BE108106]	-12.5

A total of 15,779 rat genes consistent with the quality criteria, genes in collagens, proteoglycans, tissue inhibitor of metalloproteinases, matrix metalloproteinases, interleukin-, and tumor necrosis factor-related genes are listed. IL, interleukin; MMP, matrix metalloproteinase; TIMP, tissue inhibitor of metalloproteinase; TNF, tumor necrosis factor.

ISSN: 1478-6362