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Supplementary Table 1 Expression of surface antigen by TRAP-positive mononuclear cells generated by co-culture with RA-NLC

From: Differentiation of monocytes into multinucleated giant bone-resorbing cells: two-step differentiation induced by nurse-like cells and cytokines

  

% Positivea

  

Monocytes from

TRAP-positive cells induced

TRAP-positive cells

Antigen

 

peripheral blood

from monocytes

from RA-SF

CD1a

 

1.24

0.11

0.24

CD1b

 

0.22

0.13

0.12

CD2

 

2.20

0.18

1.01

CD3

 

0.82

0.85

0.82

CD4

 

0.28

0.61

0.35

CD5

 

0.23

0.44

0.29

CD11a

LFA-1

94.72

1.16

1.18

CD11b

CR3 a chain

95.46

99.42

99.56

CD11c

CR4 a chain

99.47

99.22

99.01

CD13

 

99.44

96.15

93.87

CD14

 

95.70

99.28

99.63

CD15

Lex

22.81

0.49

15.97

CD16

FcgR III

0.72

0.24

0.30

CD19

 

0.33

0.13

0.13

CD20

 

0.36

0.11

0.10

CD34

 

0.54

0.18

0.18

CD35

CR1

93.49

0.50

0.60

CD45

 

98.73

94.28

92.90

CD45RA

 

0.37

0.15

0.46

RA45RO

 

0.65

4.18

3.30

CD51/61

VNRb

0.22

0.10

0.17

CD54

ICAM-1

0.21

0.30

0.33

CD68

 

94.59

1.06

1.10

CD80

B7/BB1

8.19

7.41

6.52

CD83

 

0.37

0.10

1.42

CD86

B70/FUN-1

90.01

0.55

1.21

HLA-A, -B, -C

 

97.44

99.68

99.53

HLA-DR

 

96.65

98.45

98.16

  1. aCells were stained with monoclonal antibody specific for various antigens, and analyzed by FACScan. Data are representative of four independent analyses. bVitronectin receptor. RA-SF = synovial fluid from patients with rheumatoid arthritis.
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Arthritis Research & Therapy

ISSN: 1478-6362

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