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Supplementary Table 2 Inhibition of formation of multinucleated giant cells by neutralizing antibodies specific for IL-3, IL-5, IL-7, and GM-CSF

From: Differentiation of monocytes into multinucleated giant bone-resorbing cells: two-step differentiation induced by nurse-like cells and cytokines

 

Fusion index (%) a

Antibodyb

IL-3 (5 ng/ml)

IL-5 (1 ng/ml)

IL-7 (20 ng/ml)

GM-CSF (1 ng/ml)

Polyclonal mouse IgG

63.8 ± 1.2

62.3 ± 3.6

66.2 ± 5.4

67.7 ± 6.0

Anti-IL-3

7.3 ± 1.6

-

-

-

Anti-IL-5

-

3.8 ± 1.8

-

-

Anti-IL-7

-

-

5.5 ± 5.8

-

Anti-GM-CSF

-

-

-

4.0 ± 1.3

  1. aFusion indices were calculated using the formula given in the Methods section. Each value is the mean ± SD of three independent experiments using TRAP-positive cells induced from monocytes of three individuals. bConcentrations of cytokines: polyclonal mouse IgG, 10 mg/ml; anti-IL-3, 10 mg/ml; anti-IL-5, 5 mg/ml; anti-IL-7, 10 mg/ml, anti-GM-CSF, 2 mg/ml. - = not examined; GM-CSF = granulocyte/macrophage-colony-stimulating factor; IL = interleukin.
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Arthritis Research & Therapy

ISSN: 1478-6362

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