Figure 2

In vitro citrullination of human fibrinogen. (a) Human fibrinogen (lane 1) was citrullinated in vitro with the recombinant hPAD2 (lane 2), hPAD4 (lane 3) and rmPAD2 (lane 4). The reaction products were analysed by SDS-PAGE and Coomassie Brilliant Blue staining (a) or by western blotting followed by detection of citrullinated proteins by anti-modified citrulline antibodies after modification of proteins on the blot (b, c). (c) Represents a longer exposure of the upper region of the blot shown in (b). The positions of the α-, β- and γ-chains of fibrinogen are indicated. Lane M contains a molecular weight marker and the molecular weights of the marker proteins are indicated on the left. In (b) lane C contains deiminated soybean trypsin inhibitor as a positive control for the detection of citrullination.