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Figure 2 | Arthritis Research & Therapy

Figure 2

From: Pathogenic effect of interleukin-17A in induction of Sjögren's syndrome-like disease using adenovirus-mediated gene transfer

Figure 2

Intracellular staining for IL-17A and IFN-γ secreting CD4+T cells in spleens of Ad5-IL17A-treated mice. Splenic leukocytes prepared from C57BL/6J mice (n = 4) at 6 wks of age (one wk prior to vector treatment) and 26 wks old (19 wks post vector treatment), considered early treatment (a-c), or splenic leukocytes prepared from C57BL/6J mice (n = 4) at 15 wks of age (1 wk prior to vector treatment) and 27 wks old (11 wks post vector treatment), considered late treatment (d-f) were examined for the presence of intracellular IL-17A and IFN-γ gated on CD4+T cells following a 5-hr in-vitro activation with anti-CD3ε and anti-CD28 in Leukocyte Activation Cocktail containing GolgiPlug. Flow cytometric acquisition was performed by staining with PE-Cy5-conjugated rat anti-CD4, FITC-conjugated rat anti-IFNγ and/or PE-conjugated rat anti-IL-17A. Data were analyzed by FCS Express. Flow cytometric images shown are from one representative analysis of two independent experiments that examined two different mice within each experiment. Data presented as mean ± SEM for n = 4 per group and statistical analyses were performed comparing the means of the Ad-LacZ and Ad-IL17A treated groups at 26 wks and 27 wks of early and late treatment, respectively. (*) indicates P < 0.5 using the Mann-Whitney U test.

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