Figure 3

Histological examination of salivary glands. Salivary gland histology was examined at 19 wks post-vector infusions of mice treated at 7 wks of age (early treatment) or at 11 wks post-vector infusions of mice treated at 16 wks of age (late treatment). Panels show representative H&E staining of salivary gland tissue from mice receiving early treatment with Ad5-LacZ (n = 10) (a), or Ad5-IL17A (n = 11) (b); fluorescent staining and enumeration of B and T cells in Ad5-IL17A treated mice (c and d) and immunohistochemical staining and enumeration of IL-17A-positive cells in Ad5-IL17A treated mice (e and f); H&E staining of salivary gland tissue from mice receiving late treatment with Ad5-LacZ (n = 10) (g), or Ad5-IL17 (n = 8) (h); and fluorescent staining and enumeration of B and T cells in Ad5-IL17A treated mice (i and j) and immunohistochemical staining and enumeration of IL-17A-positive cells in Ad5-IL17A treated mice (k and l). Black arrows indicate representative lymphocytic infiltrate.