Figure 4

CD4⁺T cells after transfection with the RFX1-siRNA expression vector pSUPER.RFX1 or pSUPER (negative control). (a) Anti-RFX1 western blot of lysates from transfected CD4⁺ T cells. Anti-β-actin Western blot is included as a loading control. (b) H3K9 tri-methylation levels at the CD11a and CD70 promoters in pSUPER.RFX1-transfected CD4⁺ T cells relative to pSUPER-transfected cells (*, P < 0.01). C: CD11a and CD70 mRNA levels in pSUPER.RFX1-transfected CD4⁺ T cells relative to pSUPER-transfected cells (*, P < 0.01). Data represent the mean ± SD of three independent experiments per group.