Cytokine levels in supernatants from human articular chondrocytes stimulated with recombinant human chemerin21-157. Cell supernatants were assessed for cytokine contents after 24 h of stimulation with 10 nM or 100 nM recombinant chemerin21-157. The levels of TNF-α (a), IL-1β (b), IL-6 (c), and IL-8 (d) are shown as mean ± standard error of the mean. Results are from six separate experiments analyzed in duplicates. Concentration is given relative to amount of protein (μg/ml). *P < 0.05, stimulated versus unstimulated.