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Table 1 Summary and sources of the assays used in this study

From: Changes in serum and synovial fluid biomarkers after acute injury (NCT00332254)

Analyte Details Assay (Reference) Sample Supplier
IL-1β Inflammatory Cytokine ELISA [8] SF, Serum R&D Systems, Minneapolis, MN
hsCRP Inflammatory marker ELISA [43] SF, Serum United Biotech, Inc, Mountain View, CA
Sulphated GAG Total proteoglycan loss (mainly aggrecan) Chemical assay SF Kamiya Biomedical Company, Seattle, WA
ARGS-aggrecan Aggrecan cleavage assay ELISA [21] SF Pfizer In house
FA846 Fetal aggrecan matrix protein (CS846 epitope) ELISA SF, Serum IBEX, Montreal, Quebec, Canada
CS846 Aggrecan epitope Competitive ELISA [43] Serum IBEX, Montreal, Quebec, Canada
C2C Collagenase neoepitope of Collagen II ELISA [43] SF, Serum IBEX, Montreal, Quebec, Canada
CTxII Collagen II degradation marker ELISA [43] SF Nordic Bioscience, Herlev, Denmark
C1,2C Type I/II collagen degradation ELISA [43] SF, Serum IBEX, Montreal, Quebec, Canada
CTxI Type I collagen degradation ELISA [44] SF, Serum Osteometer MediTech, Inc, Hawthorene, CA
NTX Bone formation marker ELISA [45] SF, Serum Osteomark, Princeton, NJ
CPII Collagen II synthesis marker ELISA [43] SF, Serum IBEX, Montreal, Quebec, Canada
Osteocalcin Bone synthesis marker ELISA [43] SF, Serum Quidel Corporation, San Diego, CA
β-Aspartate Age dependant protein modification Enzyme Assay [46] SF, Serum Promega, Madison, WI
D-Asx Age dependant protein modification HPLC [22] SF, Serum Kraus laboratory In house
D-Serine Age dependant protein modification HPLC [22] SF, Serum Kraus laboratory In house
sCD44 Cartilage marker ELISA [47] SF, Serum eBioscience, Inc, San Diego, CA
COMP Cartilage matrix protein ELISA [23] SF, Serum Kraus laboratory
Tenascin C Cartilage matrix protein ELISA [48] SF IBL International GmbH, Toronto, ON, Canada
Lubricin Cartilage matrix protein ELISA SF Pfizer In house
MMP-3 Proteolytic enzyme ELISA [49] SF, Serum R&D Systems, Inc., Minneapolis, Mn
  1. COMP, cartilage oligomeric matrix protein; CRP, C-reactive protein; GAG, glycosaminoglycan.