Changes in serum and synovial fluid biomarkers after acute injury (NCT00332254)

Catterall, Jonathan B; Stabler, Thomas V; Flannery, Carl R; Kraus, Virginia B

doi:10.1186/ar3216

Arthritis Research & Therapy

Table 2 Biomarker concentrations at baseline and follow-up in serum and synovial fluid after acute knee injury

From: Changes in serum and synovial fluid biomarkers after acute injury (NCT00332254)

	Synovial Fluid Levels			Serum Levels			Serum vs Synovial Fluid
Biomarker	Baseline Mean ±SD	Follow Up Mean ±SD	P-value	Baseline Mean ±SD	Follow Up Mean ±SD	P-value	Mean Ratio (synovial fluid/serum levels) ± SD, P-value	Correlation (between serum and synovial fluid levels), P-value
Inflammation markers
IL-1β (pg/ml)	1.02 ± 0.66	0.61 ± 0.22	P = 0.124	0.17 ± 0.06	0.24 ± 0.12	P = 0.102	↑4.3 ± 2.1, p < 0.0001	r_s = 0.358, P = 0.123
CRP (μg/ml)	1.67 ± 1.28	0.68 ± 0.64	P = 0.039	3.18 ± 2.93	1.78 ± 2.10	P = 0.102	↓3.5 ± 4.2, p = 0.012	r _s = 0.658, P = 0.002
Proteoglycan markers
GAG (μg/ml)	330.7 ± 203.2	170.2 ± 45.9	P = 0.008	---	---	---	---	---
ARGS aggrecan (μg/ml)	230.7 ± 236.7	63.2 ± 54.0	P = 0.004	---	---	---	---	---
FA846 (ng/ml)	1134.0 ± 158.1	886.5 ± 295.8	P = 0.074	31.2 ± 35.1	32.4 ± 43.8	P = 0.520	↑59.1 ± 6.3, p = 0.0001	r _s = -0.179, P = 0.464
CS846 (ng/ml)*	---	---	---	72.3 ± 139.2	58.1 ± 131.6	P = 0.413	---	---
Collagen markers
C2C (ng/ml)	184.1 ± 37.6	208.9 ± 56.3	P = 0.301	226.5 ± 34.8	216.3 ± 49.2	P = 0.700	↓1.2 ± 0.5, p = 0.232	r_s = -0.463, P = 0.046
CTxII (μg/L)	0.59 ± 0.58	1.09 ± 0.63	P = 0.012	---	---	---	---	---
C1,2C (μg/ml)	0.28 ± 0.10	0.37 ± 0.08	P = 0.039	0.53 ± 0.18	0.51 ± 0.19	P = 0.638	↓1.8 ± 1.2, p = 0.005	r_s = 0.209, P = 0.391
CTxI (ng/ml)	0.75 ± 0.39	1.23 ± 0.67	P = 0.004	0.52 ± 0.32	0.66 ± 0.31	P = 0.083	↑1.6 ± 0.5, p = 0.0002	r_s = 0.819, P < 0.0001
NTx (nM BCE)	15.58 ± 4.37	19.64 ± 7.78	P = 0.008	18.67 ± 6.92	21.6 ± 4.2	P = 0.054	↑1.2 ± 0.2, p = 0.012	r_s = 0.828, P < 0.0001
CPII (ng/ml)	308.1 ± 257.4	318.3 ± 185.1	P = 0.820	365.2 ± 181.5	288.2 ±106.8	P = 0.102	↑1.3 ± 0.9, p = 0.976	r_s = -0.102, P = 0.679
Osteocalcin (ng/ml)	15.43 ± 4.99	17.08 ± 6.16	P = 0.301	19.91 ± 8.54	16.3 ± 5.6	P = 0.032	↑1.2 ± 0.3, p = 0.012	r_s = 0.747, P = 0.0002
Post-translational markers of protein age
β-Aspartate (μM)	0.94 ± 0.32	0.76 ± 0.37	P = 0.129	---	---	---	---	---
D-Asx (D/D+L)	0.018 ± 0.001	0.019 ± 0.002	P = 0.423	---	---	---	---	---
D-Serine (D/D+L)	0.002 ± 0.001	0.003 ± 0.001	P = 0.055	---	---	---	---	---
Other Biomarkers
sCD44 (ng/ml)*	128.1 ± 49.0	105.0 ± 28.2	P = 0.250	115.9 ± 26.9	110.7 ± 39.0	P = 0.820	↑1.1 ± 0.4, p = 0.922	r_s = 0.301, P = 0.226
COMP (μg/ml)	140.9 ± 65.4	99.1 ± 32.3	P = 0.055	8.6 ± 4.1	8.0 ± 4.4	P = 0.278	↑21.2 ± 15.3, P = 0.0001	r_s = -0.326, P = 0.173
Tenascin C (ng/ml)	813.2 ± 428.6	908 ± 599	P = 0.570	---	---	---	---	---
Lubricin (nM equ)	390.4 ± 159.6	215.9 ± 59.0	P = 0.008	---	---	---	---	---
MMP-3 (ng/ml)	29.02 ± 20.84	30.89 ± 26.56	P = 0.910	0.05 ± 0.02	0.04 ± 0.02	P = 0.148	↑738.8 ± 602.5, P = 0.0001	r_s = 0.479, P = 0.038

Results represent mean ± SD data for n = 9 patients for synovial fluid values and for serum n = 11 or n = 9 for * marked biomarkers due to sample depletion. Non-parametric paired analyses by Wilcoxon Signed Rank test were performed to evaluate for significant differences between baseline and follow up time points and between serum and synovial fluid values. Non parametric Spearman Rank correlations (r_s) were performed to determine correlations between synovial fluid and serum and the ratios reported are mean ratios ± SD.

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