Association between synovial fluid levels of aggrecan ARGS fragments and radiographic progression in knee osteoarthritis

Table 2 Technical performance of the aggrecan capture OA-1 ARGS ELCL assay

Range of detection of standards	0.075 to 5 pmol ARGS/ml
Minimum required dilution of SF	1:4
Lower limit of detection in SF	0.3 pmol ARGS/ml SF
Parallelism (obtained/expected) of four SF samples diluted 1:4 to 1:4,000^a	100% (78% to 120%)^b
Spiking recovery of equimolar spiking of seven SF samples	116% (92% to 133%)^b
Intra-assay CV, n = 14	3.5%
Inter-assay CV, n = 9	16.6%

CV, coefficient of variation; ELCL, electrochemiluminescence; SF, synovial fluid. ^aSFs with 9, 95, 131, and 444 pmol ARGS/ml diluted and read within the range of detection of the standard curve. ^bMean (range).

ISSN: 1478-6362